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Test methods

 The EURLP’s diagnostic activities are performed in accordance with ISO/IEC 17025. The lack of international standards for the diagnosis of foodborne parasitic diseases is a major constraint in implementing a quality assurance system. To date, diagnostic methods have not been standardized at European or international level. Thus there is a need to validate both commercial kits and in-house developed tests. EURLP staff, on the basis of long-lasting scientific experience, developed new tests and reagents for detecting parasitic pathogens, and contributed to work up international guidelines.
EURLP developed and validated the following diagnostic methods:

"Detection of anti-Trichinella antibodies in swine serum by indirect ELISA"
"Identification...

Published 10-10-2006 in Diagnostic , last update 07-31-2014 More...

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Development and validation of analytical methods

 Trichinella spp. - Three analytical methods were developed, validated and accredited and are available as part of the services provided by the EURLP to NRLs for parasites: two ELISA test for the detection of anti-Trichinella antibodies in human and swine sera, respectively, and a Multiplex PCR method for the identification of Trichinella parasites at species level. Moreover, the magnetic stirrer method for pooled sample digestion for the detection of Trichinella in fresh meat (Commission Regulation No 2075/2005, see Section "Documents - EU Regulations"), was also accredited.
Anisakidae worms - EURLP developed, validated and accredited a PCR/RFLP method and a Multiplex PCR for the identification at species level of parasites of the family...

Published 10-10-2006 in Activities , last update 07-31-2014 More...

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