Aim of the PT: molecular identification of Echinococcus DNA at species level.
Who can participate to the PT: all the National Reference Laboratories (NRLs) for parasites from EU member states or associated countries.
PT Panel composition: the panel of samples consists of three tubes containing DNA purified from Echinococcus granulosus sensu lato (s.l.), Echinococcus multilocularis and a negative control.
Procedure to analyze the panel of samples: the EURLP recommends the use of the multiplex PCR assay developed by Trachsel and colleagues (Parasitology, 2007, 134(Pt 6): 911-20) for the identification of Echinococcus at species level. However, any other suitable molecular-based method will be accepted.
Criteria for the result evaluation: results evaluation is only qualitative. Participants have to correctly identify the two species belonging to the genus Echinococcus. Final evaluation is considered “positive” if all species are successfully identified.
Vocabolario LEU di riferimento per i parassiti
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