European Union Reference Laboratory for Parasites

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European Union Reference Laboratory for Parasites

PT-05: Detection of Echinococcus sp. worms in the intestinal mucosa of the definitive host

 

Aim of the PT: detection of worms of Echinococcus sp. in a matrix made by intestinal mucosa.

Who can participate to the PT: all the National Reference Laboratory (NRL) for parasites from EU member states or associated countries.

Procedure to analyse the panel of samples: a panel of 3 PT items is delivered to the participants. Each 13 ml sample consists of homogenized intestinal mucosa plus ethanol 70% (ratio 2:1), spiked or not with worms of Echinococcus spp. Participants are requested to analyse the items by the sedimentation and counting technique (SCT) within 7 days after the delivery to the lab.

Criteria for the result evaluation: results evaluation is only qualitative, the results of the SCT are reported “correct” or “incorrect”, irrespective of the number of worms in the sample/s. Final evaluation is considered “positive” if all spiked items are successfully detected.


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