Reference laboratories

European Union Reference Laboratory for Parasites

PT-07: Molecular identification of Anisakid nematodes at the species level

 Aim of the PT: identification of anisakid L3 larvae and DNA at species level by a molecular method.

Who can participate to the PT: all the National Reference Laboratories (NRLs) for parasites from EU member states or associated countries.

PT Panel composition: the panel of samples consists of 4 tubes, 2 tubes containing a single fragment of Anisakidae L3 larva each, and 2 tubes containing DNA extracted from a single Anisakidae L3 larva.

Procedure to analyze the panel of samples: the EURLP recommends performing the analysis using one of the EURLP methods  MI-04  or MI-10.  Any other suitable molecular method performed by the participant laboratory can be applied.

Criteria for the result evaluation: results evaluation is only qualitative. Participants have to correctly identify the larvae in the samples at species level. Final evaluation is considered “positive” if all species are successfully identified.