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13/27 - Linea guida del test dei micronuclei in Vicia faba per la valutazione di effetti mutageni in acque dolci e sedimenti.A cura di Bianca Gustavino, Silvana Caciolli e Laura Mancini 2013, ii, 32 p.
Guideline of the micronucleus test in Vicia faba for the evaluation of mutagens in freshwaters and sediments.
Edited by Bianca Gustavino, Silvana Caciolli and Laura Mancini
2013, ii, 32 p. (in Italian)
In the present guideline a standardized method is described for the execution of the MicroNucleus test (MN-test) in Vicia faba root tip cells, which represents one of the most performed test for the detection of mutagenic effects in freshwater environments, due to its high sensitivity and reliability. This guideline contains: the methodology for germination and root growth from Vicia faba seeds; the methodology for cell preparation and for microscopic analysis; basic criteria for an appropriate exposure of roots to the environmental matrices to be tested; the criteria for micronucleus identification and analysis of their frequency; other cytogenetic parameters for a correct interpretation of results and their biological/environmental significance. The use of this test is also linked to the reduction in the use of animal testing which makes it particularly suitable for monitoring of polluted environments. Therefore, the procedures, the materials and the explanatory texts on the experimental planning, provide a useful support for the training of technicians engaged for these investigations.
Key words: Micronucleus; Vicia faba; Eco-genotoxicology; Environmental mutagenesis; Water; Sediments
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