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09/39 - Protocollo preliminare di analisi microtomografica in vitro dell’interfaccia osso-impianto dentale.Rossella Bedini, Alessandra Bianco, Paolo Colangelo, Raffaella Pecci, Francesca Rizzo, Paolo Trisi, Pietro Ioppolo2009, ii, 85 p.
Preliminary protocol of in vitro microtomographic analysis of the bone-dental implant interface.
Rossella Bedini, Alessandra Bianco, Paolo Colangelo, Raffaella Pecci, Francesca Rizzo, Paolo Trisi, Pietro Ioppolo
2009, ii, 85 p. (in Italian)
The aim of this work has been bone-implant interface study, defined in literature like BIC (Bone Implant Contact) subjected to early loading testing. The implant conditions during early loading have been simulated in vitro on animal bone subjected to fatigue test by means of servo-hydraulic testing machine. According to a fatigue testing protocol set up in this work, different kind of dental implants have been tested, from Sandblasted Large-grid Acid-etched (SLA) surface treatment to no treated surface and from implant standard model to the experimental ones. A new no destructive sample analysis, named 3D microtomography, has been used to investigate before and after fatigue testing, in order to compare a sample implanted in animal bone with the same one after early loading testing. Data processing, after sample 3D reconstruction, allows qualitative and quantitative evaluating about the characteristics of bone-implant interface subjected to in vitro early loading simulation. Vertical and horizontal slices have been obtained to display the implant displacement in bone. To avoid an high level of tissue degeneration, at the end of fatigue test and microtomography analysis, each sample has been immediately glued in formalin and subjected to traditional histological analysis, more often used in bone and implant investigations.
Key words: Bone-implant interface; 3D Microtomography; Histological analysis
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