European Union Reference Laboratory for Parasites


European Union Reference Laboratory for Parasites

PT-07: Molecular identification of Anisakid nematodes at the species level

 Aim of the PT: identification of anisakid L3 larvae and DNA at species level by a molecular method.

Who can participate to the PT: all the National Reference Laboratories (NRLs) for parasites from EU member states or associated countries.

PT Panel composition: the panel of samples consists of 4 tubes, 2 tubes containing a single fragment of Anisakidae L3 larva each, and 2 tubes containing DNA extracted from a single Anisakidae L3 larva.

Procedure to analyze the panel of samples: the EURLP recommends performing the analysis using one of the EURLP methods  MI-04  or MI-10.  Any other suitable molecular method performed by the participant laboratory can be applied.

Criteria for the result evaluation: results evaluation is only qualitative. Participants have to correctly identify the larvae in the samples at species level. Final evaluation is considered “positive” if all species are successfully identified.


Proficiency testing