LOT 3

LOT 3 (Methodological and generic case studies) focuses on five methodological and cross-cutting case studies. These do not cover a full risk assessment for a specific material, rather they aims to methodological progress and guidance update.

The case studies are as follows:

1. Nanopesticides: nanocarrier loaded with a pesticide active substance
2. Nanofibres, with nanocellulose as an example
3. ‘Aging’: simulating transformations in the GI tract to obtain relevant materials for in vitro testing
4. Diseased models for the intestinal barrier to generate information on potential risks in vulnerable populations
5. Whole organism in vitro models; the bridge between in vitro models and in vivo models in nanoparticle toxicity assessment

The project coordinator is Francesco Cubadda (ISS, Italy). ISS leads case studies 2 and 3. Besides ISS, the partners are:

• German Federal Institute for Risk Assessment (BfR, Germany)
• French Agency for Food, Environmental and Occupational Health & Safety (ANSES, France)
• Sciensano (Belgium)
• National Institute for Public Health and the Environment (RIVM, The Netherlands)
• Food Safety Research, Wageningen Research Foundation (WFSR, The Netherlands)
• Luxembourg Institute of Science and Technology (LIST, Luxembourg)
• Universiteit Hasselt (Belgium)
• Universitat Politècnica de València (UPV, Spain)

The Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM, Germany) and the University of Auckland (New Zealand) contribute as subcontractors to specific case studies. The European Commission Joint Research Centre (EC-JRC, Italy) will contribute to the project as external partner.